L-Tryptophan as Fluorescent Probe for Determination of Folic Acid in Pharmaceutical Products

Tahir, Tara F. and Qader, Aryan F. and Salih, Musher I. and Rashid, Essa Q. (2019) L-Tryptophan as Fluorescent Probe for Determination of Folic Acid in Pharmaceutical Products. ARO-THE SCIENTIFIC JOURNAL OF KOYA UNIVERSITY, 7 (2). pp. 19-26. ISSN 2410-9355

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Abstract

A new fluorescent probe L-Tryptophan was reported for the determination of folic acid (FA), based on its quenching effect of the fluorescence intensity of L-Tryptophan. The concentration of folic acid was proportional to the quenched fluorescence intensity of L-Tryptophan at an emission wavelength of 365 nm in Britton– Robinson (BR) buffer solution of pH 7. Optimized conditions of pH, time, order of addition of the reagent, potential interferences, concentrations of L-Tryptophan and buffer were investigated. Folic acid was determined in a linear range of 2.0 to 16.0 μg ml-1 with a correlation coefficient R2 0.9974. The limits of detection LOD and quantification LOQ values were 0.09 μg ml-1 and 0.27 μg ml-1, respectively. The standard deviation (RSD) values for five replicated measurements of 2, 8, 16 μg ml-1 folic acid were between 0.23 % and 1.07%. This method is efficient for routine analysis and quality control assay as it is relatively interferences free.

Item Type:	Article
Uncontrolled Keywords:	Folic acid, L-Tryptophan, Pharmaceutical tablet, Spectrofluorimetry
Subjects:	Q Science > QD Chemistry
Divisions:	ARO-The Scientific Journal of Koya University > VOL 7, NO 2 (2019)
Depositing User:	Dr Salah Ismaeel Yahya
Date Deposited:	19 Mar 2020 20:03
Last Modified:	30 Mar 2020 22:34
URI:	http://eprints.koyauniversity.org/id/eprint/198

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