Identification DNA Methylation Change of ABCC8 Gene in Type 2 Diabetes Mellitus as Predictive Biomarkers

Smail, Harem O. and Mohamad, Dlnya A. (2022) Identification DNA Methylation Change of ABCC8 Gene in Type 2 Diabetes Mellitus as Predictive Biomarkers. ARO-THE SCIENTIFIC JOURNAL OF KOYA UNIVERSITY, 10 (1). pp. 63-67. ISSN 2410-9355

Text (PDF File) ARO.10947-Vol10.No1.2022.ISSUE18-PP 63-67.pdf Available under License Creative Commons Attribution Non-commercial Share Alike. Download (1MB)

Abstract

Type 2 diabetes mellitus is the most common chronic endocrine disorder that affecting 5%–10% of adults globally. Recently, the disease has rapidly spread throughout the Kurdistan Region. This study investigates DNA methylation status in the ABCC8 gene among the study population, and it possibly used as a biomarker. One hundred and thirteen individuals were included in this study, and they were divided into three categories (47 diabetes, 36 prediabetic, and 30 controls). Blood samples were collected to investigate DNA methylation status in patients who attended private clinical sectors in Koya city, Kurdistan Region of Iraq, between August and December 2021. Methylation-specific PCR (MSP) uses paired primers for each methylated and unmethylated region. In addition, the X2 Kruskal–Wallis statistical and Wilcoxon signed-rank tests were run with a significance level of p 0.05. In comparison to the healthy group, hypermethylation of DNA is detected in the promoter region of diabetes and prediabetes. In addition, age, gender, BMI, alcohol use, family history, and physical activity all influence the degree of DNA methylation in people who have had coronavirus illness. The abovementioned findings suggest that DNA methylation alterations in the ABCC8 promoter region might be exploited as a possible predictive biomarker for type 2 diabetes mellitus diagnosis.

Item Type:	Article
Uncontrolled Keywords:	ABCC8 gene, Diabetes mellitus, DNA methylation, Methylated primer, Unmethylated primer
Subjects:	Q Science > QR Microbiology
Divisions:	ARO-The Scientific Journal of Koya University > VOL 10, NO1 (2022)
Depositing User:	Dr Salah Ismaeel Yahya
Date Deposited:	05 Aug 2022 12:05
Last Modified:	05 Aug 2022 12:05
URI:	http://eprints.koyauniversity.org/id/eprint/315

Actions (login required)

View Item